(PDF) Culture of IMR90 Cells - ResearchGate

Catalog of Stem Cell Lines / iPS (IMR90)-4 iPS (IMR90)-4 Derived from IMR90 …

Abstract Oncogene-induced senescence (OIS) is a cellular response that limits the replication of cells expressing oncogenes. As a result, OIS is a potent tumor suppressor mechanism limiting cancer progression. Here we describe IMR90 ER:RAS, a widely used model to study OIS in cell culture. G IMR90 cells were infected with an empty pGIPZ vector (vector) or shRNA targeting either CASP4 (shC4), GSDMD (shGSDMD) or TP53 (shP53) … Jul 23, 2018 and IMR90-4 hiPSC lines, respectively, with and without transient puromycin selection. For EP1 . CC-BY 4.0 International license. Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells Junying Yu,1,2* Maxim A. Vodyanik,2 Kim Smuga-Otto,1,2 Jessica Antosiewicz-Bourget,1,2 Jennifer L. Frane,1 Shulan Tian, 3Jeff Nie,3 Gudrun A. Jonsdottir, Victor Ruotti, Ron Stewart,3 Igor I. Slukvin,2,4 …

One additional enrichment of iPS-RPE from iPS (IMR90)-4 was performed after 8 months in bFGF-free culture, specifically to examine the … IMR90-4 iPSCs were first differentiated for 6 days in unconditioned medium (UM) to generate a mixed population of neural cells and … Cell Line Alias, iPS(IMR90) clone (#4). Cell Type, Human iPS. Disease, None reported. Sex, Female. Genetically Related Cell Lines, No. IMR90-4 (WiCell Research Institute, Inc.) This induced pluripotent stem cell (iPSC) line was generated from IMR90 fetal lung fibroblasts by viral transduction of a … 0hr_IMR90_4: Sample type: RNA : Source name: IMR90 cells, 0uM, 0 hour: Organism: Homo sapiens: Characteristics: cell type origin: fibroblast transcription …

iPSIMR90-4 - WiCell

Cerebral hypoxia/ischemia (H/I) is an important stress factor involved in the disruption of the blood–brain barrier (BBB) following stroke injury, … IMR90-4-derived BMECs were cultured alone (monoculture) or co-cultured with either rat astrocytes or human embryonic kidney (HEK) cells for 96 hours and trans-endothelial electrical resistance (TEER) was monitored. Error bars represent standard deviation of triplicate filters. To this aim, we induced aneuploidy in IMR90 human primary cells by depleting pRB, DNMT1 and MAD2 and analyzed their gene expression profiles by microarray analysis. Bioinformatic analysis revealed a common gene expression profile of IMR90 cells that became aneuploid.

Human Fibroblast IMR90 Hi-C Data Dixon et al.

Imr90 4 Dl1 Ips Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > ATCC > imr90 4 dl1 ips cells. imr90 4 … | Morphology of iPS(IMR90)-4 cell spheroids with the initial cell number of 500 and 1,000 cells per spheroid at the undifferentiated stage (3D SC) and at days 2
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Each iPS(IMR90) clone expressed telomerase activity and the human ES cell–specific cell surface antigens SSEA-3, SSEA-4, Tra-1-60, and Tra-1-81 , whereas the parental IMR90 cells did not. Microarray analyses of gene expression of the four iPS(IMR90) clones confirmed a similarity to five human ES cell lines (H1, H7, H9, H13, and H14) and a Cellosaurus IPS (IMR90)-4 (CVCL_C437) From: University of Wisconsin; Madison; USA. Omics: Transcriptome analysis. Derived from sampling site: Fetal lung. Cell type=Fibroblast. Induced pluripotent stem cell lines derived from human somatic cells. Growth factor-activated stem cell circuits and stromal signals cooperatively accelerate non Senescent IMR90 have an average of 4.0 and 4.4 times as much ceramide relative to total phospholipid as do young IMR90 that were made quiescent by serum deprivation or high cell density, respectively . The difference is significant for both serum-deprived (p = .029) and non-serum-deprived (p = .017) senescent cells. Inducible ER:Ras IMR90 cells were generated using the vector pLNC-ER:RasV12 and treated with 1μM 4-hydroxy-tamoxifen for Ras induction. Quiescent IMR90 were obtained by culturing the cells in serum-free medium for 48 hours. For MEK inhibition, PD98059 was added during retroviral transduction at a final concentration of 20μM. S enescence in primary human fibroblasts (IMR90 cells) can be delayed by several mitochondrial protective compounds: carnosine (), aminoguanidine (), and N-t-butyl hydroxylamine (NtBHA) (3, 4… The H9 and IMR90-4 cell lines were obtained via a WiCell agreement (H9: NO.16-W0060; IMR90-4: NO.17-W0063); DS1, 2DS3, DSP, and DS2U … The combined expression of endogenous and exogenous pluripotency markers [the later derived from viral vectors containing NANOG and OCT3/4 for iPS(IMR90)

Aneuploid IMR90 cells induced by depletion of pRB, DNMT1

The homeoprotein SIX1 controls cellular senescence through ...

Cellosaurus IPS (IMR90)-4 (CVCL_C437) From: University of Wisconsin; Madison; USA. Omics: Transcriptome analysis. Derived from … Figure 4. Tetraploidy increases in the course of IMR90 senescence. (a) The number of hypertetraploid cells increases with senescence and strongly inversely Product Name iPS(IMR90)-4 Alias iPS(IMR90) clone (#4) Lot Number WB0088 Parent Material iPS(IMR90)-4-MCB-01 Depositor University of Wisconsin – Laboratory of Dr. James Thomson Banked by WiCell Thaw Recommendation Thaw 1 vial into 4 wells of a 6 well plate. IMR90-4, and H9) at day 3, 7, and10 after plating (Fig-ure 1E). These cellular deﬁcits were replicated by high-con-tent measurement (Figure 1F) whereby … Combining Brain-1 R1/R2 and IMR90-1 R1/R2 technical replicates together (so-called “low-coverage” libraries, ∼9.4 and ∼9 M reads, … Cells were incubated at 4 °C overnight with primary antibodies (Supplementary IMR90-4 lung. OCT3/4, SOX2,. LIN28, KLF4 lentiviral. Cellosaurus IMR-90 (CVCL_0347) Part of: ENCODE project common cell types; tier 2. Part of: International Histocompatibility Workshop cell lines. …